Synthetic Biologist position Cambridge Glycoscience

Applications are invited from talented and passionate scientists for a position leading Cambridge
Glycoscience’s synthetic biology R&D programme.

Cambridge Glycoscience is a food technology start-up. Our mission is to tackle the greatest public health crisis of modern times – excessive sugar consumption. We do this by creating functional, nutritional, and economic for sugar that function across the food industry, using cutting edge synthetic biology approaches. We operate across the value chain from lab bench and industrial production all the way to the kitchen and consumers. We have strong connections to the University of Cambridge’s Biochemistry Department. 

Event report: Glycobiotechnology 2018

Glycobiotechnology 2018 took place on Monday 3rd and Tuesday 4th September 2018 in Manchester and was attended by approx. 180 IBCarb members from academia and industry.

Glycobiotechnology is an essential facet of modern industry, with broad applications in Medicine such as Biopharmaceuticals and Vaccines, Healthy Food and Renewable Biomaterials. This 2-day event celebrated advances made in glycobiotechnology over the past five years through open academic-industry partnerships within the IBCarb network. The meeting brought together professionals, academics and students working in the area of glycobiotechnology and showcased the latest developments and discussed future opportunities in glycoscience and industrial biotechnology.

Also highlighted during the meeting were the RSC Carbohydrate group and CarboMet other initiatives everyone was strongly encouraged to get involved with.

The Complex life of Sugars outreach stand was exhibited during the event. At the end of day 1, the Sweet Existence artwork was also exhibited with the artist Karen Barber in attendance.

We would like to thank everyone who participated in the event, and especially all our speakers and session chairs for delivering such a superb range of talks and discussions.

Full list of speakers and talks at the event (in presentation order):

Joachim Thiem (Glycom & Universität Hamburg) ‘Chemoenzymatic Syntheses and Inhibition Studies’

Simon Webb (University of Manchester) ‘Enzymatic Modification of Synthetic Glycolipids in Vesicle Membranes’

Jerry Turnbull (University of Liverpool) ‘Chemoenzymatic strategy for generating next-generation heparin therapeutics’

Ed Yates (University of Liverpool) ‘Seaweeds as a source of bioactive materials for wound healing’

Igor Baruskov (University of Liverpool) ‘Identification and evaluation of algal sulfotransferases for enzymatic polysaccharide modifications’

BBSRC overview – Elizabeth Saunders, Strategy & Policy Manager

Stuart Haslam (Imperial College London)  ‘Structural Glycobiology for Glycobiotechnology’

Bruce Turnbull (University of Leeds)  ‘Lectin Engineering’

Gerd Wagner (Kings College London)  ‘Chemical Glycoengineering of IgE Antibodies’

Andrew Powell (Liverpool John Moore University) ‘Investigating Skin-Antibiotic Activities of Heparin: Priming Heparin-Based Industrial Biotechnology’

Barbara Pfister (ETH Zurich) ‘Recreating Starch Synthesis in Yeast’

Dani Ungar (University of York) ‘Modelling Glycan Processing Reveals Golgi-Enzyme Homeostasis in Different Cellular States’

Graham Stafford (University of Sheffield) ‘Oral microbiology and glycoenzymes’

Andrew Laws (Huddersfield University) ‘Probiotic Polysaccharides’

Federico Sabbadin (University of York) An Ancient Family of Lytic Polysaccharide Monooxygenases with Roles in Arthropod Development and Biomass Digestion’

Frederique Lisacek (Swiss Institute of Bioinformatics) ‘Glycoinformatics: A Toolbox for Bridging Glycoscience with Multiple – Omics’

Vincent Bulone (KTH Royal Institute of Technology) ‘Structural diversity and biosynthesis of fungal and oomycete cell wall polysaccharides’

Gavin Miller (Keele University) ‘Building blocks for Designer Alginates’

Martina Lahmann (Bangor University) ‘Synthesis of C-glycoconjugates as tools for biological investigations’

Matthew Gibson (Warwick University) ‘Glycosylated Nanomaterials; Design and Application’

Paul Dupree (University of Cambridge)  ‘Detailed enzymic characterization of Gum Odina polysaccharide, and scale up of components for later studies of functionality’

Eleanor Binner (University of Nottingham) ‘Microwave-assisted depolymerisation of an algal polysaccharide’

Nathalie Juge (Quadram Institute Bioscience) ‘Biological role and application of intramolecular trans-sialidases from gut symbionts’

David Green (Scottish Association for Marine Science) ‘Identifying novel enzymes for the modification of a marine algal polysaccharide’

Seung Lee (University of Southampton) ‘Development of hyper-activity glycosynthase for high value glycosides’

Darren Smith (Northumbria University) ‘Metagenomics as a tool for the rapid discovery of novel glycosyltransferases’

RuAngelie Edrada-Ebel (University of Strathclyde) ‘Development of high-value compounds from Bacillus licheniformis’

Timothy Douglas (University of Lancaster) ‘Mineralized polysaccharide hydrogel biomaterials for bone tissue regeneration’

Adélaïde Raguin (Heinrich Heine University Düsseldorf) ‘Stochastic modeling of starch granule biogenesis’

Jolanda van Munster (University of Manchester) ‘Sweet spots for fungal lignocellulose degradation; Surface analysis tools to identify activity of Aspergillus niger and its enzymes on plant biomass’

PhD positions Marie-Curie ITN ‘NanoCarb’

2 vacancies as part of the Marie-Curie Innovative Training Network ‘NanoCarb’ are available in the lab of Professor Matt Gibson at Warwick University. This is a trans-european collaboration to investigate biomedical application of glycosylated nanomaterials and will have signifcant travel and international collaboration opportunities with academics and industry.

Position 1. Glyco and polymer chemistry. Synthetic-based PhD to undertake the synthesis of glyconanoparticles and the biophysical testing of their function. Chemistry background needed.

Position 2. Recombinant production of carbohydrate-binding proteins and investigation of their function. Biology/Biochemistry Background.

There are strict Mobility Criteria for these positions; At the time of recruitment, the applicant must not have resided or carried out his/her main activity (work, studies, etc…) in the country of the host institution (employer) for more than 12 months in the 3 years immediately prior to his/her recruitment under the project. Compulsory national service and/or short stays such as holidays are not taken into account.

Contact Professor Matt Gibson, including your CV if you want more information m.i.gibson@warwick.ac.uk

 

PhD positions Marie-Curie ITN ‘PoLiMeR’

PhD positions available in experimental and computational sciences for the
Marie-Curie Innovative Training Network “PoLiMeR’ Polymers in the Liver – Metabolism & regulation’.

PhD student 1: Kinetic analysis of enzymes in glycogen metabolism
Host organisation: John Innes Centre, Norwich UK*
Contact: Professor Rob Field (Rob.Field[at]jic.ac.uk)

PhD student 2: Structural analysis of glycogen from in vitro models and patients with Glycogen Storage Diseases
Host organisation: Iceni Diagnostics, Norwich UK*

Contact: Dr Stephan Goetz (stephan.goetz[at]icenidiagnostics.com)

See here for details and to apply.

Glycobiotechnology 2018 – final program

Glycobiotechnology 2018 is taking place in Manchester on 3rd & 4th September 2018.

See here for more details. The full program is below.

Day 1 – Monday 3rd September 2018

9.30 -10.30 Arrival, Registration & Refreshments

Session 1 Chair: Sabine Flitsch, University of Manchester

10.30 – 10.40 Welcome & Introduction – Sabine Flitsch – IBCarb Director

10.40 – 11.10 Invited Speaker: Joachim Thiem (Glycom & Universität Hamburg) ‘Chemoenzymatic Syntheses and Inhibition Studies’

11.10 – 11.25 Short talk: Simon Webb (University of Manchester) ‘Enzymatic Modification of Synthetic Glycolipids in Vesicle Membranes’

11.25 – 11.40 Short talk: Jerry Turnbull (University of Liverpool) ‘Chemoenzymatic strategy for generating next-generation heparin therapeutics’

11.40 – 11.55 Short talk: Ed Yates (University of Liverpool) ‘Seaweeds as a source of bioactive materials for wound healing’

11.55 – 12.10 Short talk: Igor Baruskov (University of Liverpool) ‘Identification and evaluation of algal sulfotransferases for enzymatic polysaccharide modifications’

12.10 – 12.25 BBSRC overview – Elizabeth Saunders, Strategy & Policy Manager

12.25 – 13.55 Lunch

Session 2 Chair: Nathalie Juge, Quadram Institute Bioscience

13.55 – 14.10 Short talk: Stuart Haslam (Imperial College London) ‘Structural Glycobiology for Glycobiotechnology’

14.10 – 14.25 Short talk: Bruce Turnbull (University of Leeds) ‘Lectin Engineering’

14.25 – 14.40 Short talk: Gerd Wagner (Kings College London) ‘Chemical Glycoengineering of IgE Antibodies’

14.40 – 14.55 Short talk: Andrew Powell (Liverpool John Moore University) ‘Investigating Skin-Antibiotic Activities of Heparin: Priming Heparin-Based Industrial Biotechnology’

14.55 – 15.25 Invited speaker: Barbara Pfister (ETH Zurich) ‘Recreating Starch Synthesis in Yeast’

15.25 – 16.10 Refreshments & Networking

Session 3 Chair: Stuart Haslam, Imperial College London

16.10 – 16.25 CarboMet Horizon 2020 project overview – Sabine Flitsch, Coordinator

16.25 –16.40 Short talk: Dani Ungar (University of York) ‘Modelling Glycan Processing Reveals Golgi-Enzyme Homeostasis in Different Cellular States’

16.40 – 16.55 Short talk: Graham Stafford (University of Sheffield) ‘Oral microbiology and glycoenzymes’

16.55 – 17.10 Short talk: Andrew Laws (Huddersfield University) ‘Probiotic Polysaccharides’

17.10 – 17.25 Short talk: Federico Sabbadin (University of York) ‘An Ancient Family of Lytic Polysaccharide Monooxygenases with Roles in Arthropod Development and Biomass Digestion’

17.25 – 17.55 Invited Speaker: Frederique Lisacek (Swiss Institute of Bioinformatics) ‘Glycoinformatics: A Toolbox for Bridging Glycoscience with Multiple –Omics’

17.55 – 18.05 Closing remarks day 1

Evening reception

18.05 – 20.00 Networking buffet & ‘Sweet Existence’ art exhibition with Karen Barber

Day 2 – Tuesday 4th September

Session 4 Chair: Steve Eichhorn, University of Bristol

9.00-9.05 Welcome to day 2

9.05 – 9.35 Invited speaker: Vincent Bulone (KTH Royal Institute of Technology) ‘Structural diversity and biosynthesis of fungal and oomycete cell wall polysaccharides’

9.35 – 9.50 Short talk: Gavin Miller (Keele University) ‘Building blocks for Designer Alginates’

9.50 – 10.05 Short talk: Martina Lahmann (Bangor University) ‘Synthesis of C-glycoconjugates as tools for biological investigations’

10.05 – 10.20 Short talk: Matthew Gibson (Warwick University)  ‘Glycosylated Nanomaterials; Design and Application’

10.20 – 10.35 RSC Carbohydrate Group overview – Matt Gibson, Chair

10.35 – 11.20 Refreshments & Networking

Session 5 Chair: Bruce Turnbull, University of Leeds

11.20 – 11.35 Short talk: Paul Dupree (University of Cambridge)  ‘Detailed enzymic characterization of Gum Odina polysaccharide, and scale up of components for later studies of functionality’

11.35 – 11.50 Short talk: Eleanor Binner (University of Nottingham) ‘Microwave-assisted depolymerisation of an algal polysaccharide’

11.50 – 12.05 Short talk: Nathalie Juge (Quadram Institute Bioscience) ‘Biological role and application of intramolecular trans-sialidases from gut symbionts’

12.05 – 12.20 Short talk: David Green (SAMS) ‘Identifying novel enzymes for the modification of a marine algal polysaccharide’

12.20 – 12.35 Short talk: Seung Lee (University of Southampton) ‘Development of hyper-activity glycosynthase for high value glycosides’

12.35 – 14.05 Lunch

Session 6 Chair: Rob Field, John Innes Centre

14.05 – 14.20 Short talk: Darren Smith (Northumbria University) ‘Metagenomics as a tool for the rapid discovery of novel glycosyltransferases’

14.20 – 14.35 Short talk: RuAngelie Edrada-Ebel (University of Strathclyde) ‘Development of high-value compounds from Bacillus licheniformis’

14.35 – 14.50 Short talk: Timothy Douglas (University of Lancaster) ‘Mineralized polysaccharide hydrogel biomaterials for bone tissue regeneration’

14.50 – 15.05  Short talk: Adélaïde Raguin (Heinrich Heine University Düsseldorf) ‘Stochastic modeling of starch granule biogenesis’

15.05 – 15.35 Invited speaker: Jolanda van Munster (University of Manchester)  ‘Sweet spots for fungal lignocellulose degradation; Surface analysis tools to identify activity of Aspergillus niger and its enzymes on plant biomass’

15.35 – 15.45 Closing remarks day 2

15.45 – 16.30 Refreshments & Networking

2020 Biochemical Society Awards now open

Do you know of an outstanding bioscientist who deserves recognition?

Nominations have now opened for the 2020 Biochemical Society Awards – the field’s most prestigious awards that recognize established researchers as well as scientists in the early stages of their career.

Nominations are welcomed from across the UK and overseas until 6pm (GMT) on Thursday 31 January 2019.

Now in their 58th year, the Society will present 12 awards in 2020, recognizing excellence and achievement in both specific and general fields of science.

This year’s results will be announced in April 2019.

All of our award prize and medal lectureships carry prize money and all award winners will be invited to submit an article to a Society-owned publication.

Further information is available online.

EU Consultation on plastics feedstocks

Stakeholders’ consultation – Comparative LCA of alternative feedstocks for plastics production

In January 2018,  the Commission adopted the European Strategy for Plastics in a Circular Economy, proposing a vision where alternative materials and feedstocks are developed and used where evidence shows they are more sustainable compared to non-renewable alternatives.

The Commission is now conducting a data gathering exercise on the life cycle of plastics and alternatives feedstocks to understand what the implications of moving away from non-renewables would be. Deadline for input is 31 July 2018.

If you are interest contact JRC-LCA4PLASTICS@ec.europa.eu

Find more information on the feedback required here: Call for data and information-Plastics LCA

Glycobiotechnology 2018 Register now!

Glycobiotechnology 2018 

Where: University Place, University of Manchester, 176 Oxford Road, Manchester, M13 9PL

When: Monday 3rd September 2018 10.00am until Tuesday 4th September 2018, 4pm

Register here

About: Glycobiotechnology is an essential facet of modern industry, with broad applications in Medicine such as Biopharmaceuticals and Vaccines, Healthy Food and Renewable Biomaterials. This 2-day event will celebrate advances made in glycobiotechnology over the past five years through open academic-industry partnerships within the IBCarb network. The meeting will bring together 200 professionals, academics and students working in the area of glycobiotechnology to showcase latest developments and discuss future opportunities in glycoscience and industrial biotechnology.

Confirmed speakers: Igor Baruskov, University of Liverpool; Eleanor Binner, University of Nottingham; Vincent Bulone, University of Adelaide & Royal Institute of Technology (KTH, SE); Paul Dupree, University of Cambridge; RuAngelie Edrada-Ebel, University of Strathclyde; Matthew Gibson, University of Warwick; David Green, Scottish Association for Marine Science; Stuart Haslam, Imperial College London; Nathalie Juge, Quadram Institute Bioscience; Martina Lahmann, Bangor University; Andrew Laws, University of Huddersfield; Seung Lee, University of Southampton; Frederique Lisacek, Swiss institute of Bioinformatics; Gavin Miller, Keele University; Elaine O’Reilly, University of Nottingham; Barbara Pfister, ETH Zurich; Darren Smith, Northumbria University; Graham Stafford, University of Sheffield; Joachim Thiem, Glycom co-founder and University of Hamburg; Bruce Turnbull, University of Leeds; Jerry Turnbull, University of Liverpool; Jolanda van Munster, University of Manchester; Gerd Wagner, King’s College London; Simon Webb, University of Manchester; Ed Yates, University of Liverpool.

Outline program:

Day 1: Monday 3rd September 2018

09:30-10:30: Registration

10:30 – 17:30: Talks (includes lunch and refreshment breaks).

17:30: Networking finger buffet & wine reception plus exhibition of the Sweet Existence artwork with the artist Karen Barber

Day 2: Tuesday 4th September 2018

09:00 – 16:00: Talks continued (includes lunch and refreshment breaks).

Register here

Industry exhibits are welcome. Please contact Claire.doherty@manchester.ac.uk directly to arrange.

Accommodation will be provided for IBCarb members*. You will need to book this on your behalf and we will reimburse you the costs after the event. Costs up to £120 max will be refunded via an expenses claim form which can be downloaded here. Completed forms should be sent to the IBCarb network manager after the event and not later than 5th October 2018.

There are a number of hotels in Manchester city centre which is within walking distance of the meeting venue. See here: https://www.visitmanchester.com/where-to-stay

Here is a list of nearby hotels, by distance, to the meeting venue and/or Piccadilly train station:

  • Crowne Plaza, Booth Street West
  • Holiday Inn, Oxford Road
  • Travelodge Manchester, Upper Brook Street
  • The Pendulum, Sackville Street
  • Hotel IBIS, Princess Street
  • Motel One, London Road
  • Macdonald Manchester Hotel, London Road

* Due to BBSRC rules you will only eligible for free registration and accommodation if you are a member of IBCarb at the time of the event. Membership of the network is free of charge. Please see http://ibcarb.com/membership/ for registration and T&Cs.

Travel bursaries of up to £200 are available for PhDs and PDRAs attending this event. Visit ibcarb.com/funding for details on how to apply.

The IBCarb management team & organising committee:

Professor Sabine Flitsch, University of Manchester & IBCarb Director

Professor Rob Field, John Innes Centre & IBCarb Co-Director

Dr Timothy Miller, Croda Europe Ltd

Dr Stuart Haslam, Imperial College London

Professor Stephen Eichhorn, University of Bristol

Dr Cleo Kontoravdi, Imperial College London

Professor Nathalie Juge, Quadram Institute, Norwich

Dr Sarah Allman, Reading University

Congratulations to Ludger & Imperial College on their joint publication

A successful Business Interaction Voucher funded by IBCarb was used to strengthen the glycomics collaboration between lmperial College London and Ludger Ltd. This has resulted in publishing the  article titled “Towards automation of glycomic profiling of complex biological materials” by Shubhakar et al. in the Glycoconjugate journal.

This paper compares the performance of an automated plate based method for N-glycan release and permethylation of glycans derived from mouse lung and kidney tissues to established standard glycomic protocols using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The study revealed that the automated workflow is highly repeatable and it shows highly comparable MALDI-TOF-MS N- and O-glycan profiles of complex heterogeneous glycoproteins when compared with the standard protocol.

The key advantages of using the automated sample processing steps include reduced sample preparation times, low sample volumes needed for processing, it eliminates the need for performing the labour intensive steps, minimises hands-on-time, increases sample throughput and this set up has also shown immense potential for multiplexing, making the automated samples processing method convenient, scalable, fast and reproducible.

Ludger’s LT-PERMET-96 kit was used for derivatising these complex biological samples. For more information on the LudgerTag Permethylation kits and to view the presentation on our technology, please visit their website here.

Reference:

Shubhakar, A., Pang, PC., Fernandes, D.L. et al. Towards automation of glycomic profiling of complex biological materials J (2018) 35: 311. https://doi.org/10.1007/s10719-018-9825-8