A successful Business Interaction Voucher funded by IBCarb was used to strengthen the glycomics collaboration between lmperial College London and Ludger Ltd. This has resulted in publishing the article titled “Towards automation of glycomic profiling of complex biological materials” by Shubhakar et al. in the Glycoconjugate journal.
This paper compares the performance of an automated plate based method for N-glycan release and permethylation of glycans derived from mouse lung and kidney tissues to established standard glycomic protocols using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The study revealed that the automated workflow is highly repeatable and it shows highly comparable MALDI-TOF-MS N- and O-glycan profiles of complex heterogeneous glycoproteins when compared with the standard protocol.
The key advantages of using the automated sample processing steps include reduced sample preparation times, low sample volumes needed for processing, it eliminates the need for performing the labour intensive steps, minimises hands-on-time, increases sample throughput and this set up has also shown immense potential for multiplexing, making the automated samples processing method convenient, scalable, fast and reproducible.
Ludger’s LT-PERMET-96 kit was used for derivatising these complex biological samples. For more information on the LudgerTag Permethylation kits and to view the presentation on our technology, please visit their website here.
Shubhakar, A., Pang, PC., Fernandes, D.L. et al. Towards automation of glycomic profiling of complex biological materials J (2018) 35: 311. https://doi.org/10.1007/s10719-018-9825-8